Antioxidant capacity of fresh and stored breast milk: Is -80°C optimal temperature for freeze storage
Journal of Maternal-Fetal and Neonatal Medicine, cilt.25, sa.6, ss.777-782, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 25 Sayı: 6
- Basım Tarihi: 2012
- Doi Numarası: 10.3109/14767058.2011.592230
- Dergi Adı: Journal of Maternal-Fetal and Neonatal Medicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.777-782
- Anahtar Kelimeler: Breast milk, Oxidative status, Stages of lactation
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
Objective: To determine total antioxidant capacity and total oxidation status in fresh and freeze stored (at -80°C) breast milk during the stages of lactation. Methods: Samples of colostrum, transitional and mature milk were collected from 44 healthy women at 3, 8 and 30 days after birth. The total milk volume collected (6ml) was divided in two aliquot parts: 3ml for the fresh analysis which was done immediately after the extraction and 3ml for storage under freezing conditions at -80°C for two months. The antioxidant status and oxidative stress of the fresh and stored breast milk were assessed via determination of total antioxidant capacity and total oxidation status. Results: Antioxidant capacity of transitional and mature milk decreased (p=0.0001, p=0.028, respectively); however, antioxidant capacity of colostrum did not change by storage at -80°C (p>0.05). Total antioxidant capacity of fresh and stored breast milk significantly decreased during the stages of lactation (p<0.0001, p=0.028, respectively). Total oxidation status showed no significant difference in fresh and stored breast milk during the stages of lactation (p>0.05). Conclusion: Freeze storage of breast milk at -80°C for two months seems not to be the optimal condition to preserve the antioxidant capacity of breast milk. © 2012 Informa UK, Ltd.