Integrating enzymatic hydrolysis and nanoparticle catalysis for sustainable bioethanol production from pumpkin and dragon fruit pomace by non-conventional yeasts based fermentation prosesses
World journal of microbiology & biotechnology, cilt.42, sa.2, ss.84, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 42 Sayı: 2
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11274-026-04818-z
- Dergi Adı: World journal of microbiology & biotechnology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, ABI/INFORM, BIOSIS, Compendex, Environment Index, MEDLINE
- Sayfa Sayıları: ss.84
- Anahtar Kelimeler: Dragon fruit pomace, Nanoparticles, Pumpkin pomace, Yeast
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
This study investigated the effects of metal oxide nanoparticles on bioethanol production from lignocellulosic wastes-dragon fruit pomace, and pumpkin pomace-using yeast strains Saccharomyces cerevisiae, Kluyveromyces marxianus, and Candida boidinii. Among the tested substrates, pumpkin pomace yielded the highest ethanol concentrations, particularly at the highest biomass loading (150 g/L). Enzymatic hydrolysis with cellulase was optimized, with 60 FPU/g substrate identified as the most cost-effective loading for maximizing sugar release and ethanol yield. The application of metal oxide nanoparticles (ZnO, Fe2O3, and NiO) was explored to enhance fermentation efficiency. NiO nanoparticles at 20 mg/100 mL significantly improved bioethanol production. Without supplementation, 21.93 g/L of bioethanol (YP/S max: 0.24 g/g, Qp max: 0.18 g/L.h) was obtained from 150 g/L PP. However, when 20 mg/100 mL NiO nanoparticles were added to a 150 g/L pumpkin pomace medium, and the enzyme loading was adjusted to 60 FPU/g substrate, the ethanol concentration increased by 95.8% to 42.64 g/L. YP/S max and Qp max were found to be 0.40 g/g and 0.89 g/L.h, respectively, in these conditions. These results demonstrate that integrating nanoparticle-assisted hydrolysis and fermentation is an effective, cost-saving approach to enhance bioethanol production from low-value agricultural residues, providing a promising approach for sustainable biofuel generation.