Zoledronic acid has been shown to inhibit cancer cell proliferation, metastasis and induce cancer cell apoptosis. Stromal Derived Factor-1 (SDF-1) is a chemokine that has been reported to be expressed markedly high in the most common metastatic sites. The aim of this study was to investigate the effect of zoledronic acid on SDF-1 mRNA expression of rats' tissues. Rats were randomised into two groups, each containing five rats. Zoledronic acid was administered 0.1 mg/kg subcutaneously to one group. Other group was used as the control group. Seven days later, all rats were sacrificed. Levels of SDF-1 mRNA expression in tissues were measured quantitatively with RT-PCR. The median of SDF-1 mRNA relative expression in the lung was 0.80 (0.49-1.00) in the control group and 0.90 (0.61-1.42) in the zoledronic acid group (p=0.53). The median of SDF-1 mRNA relative expression in the liver was 0.90 (0.39-1.00) in the control group and 0.93 (0.77-1.29) in the zoledronic acid group (p=0.35). In this study, we investigated the effect of zoledronic acid on the tissue SDF-1 mRNA expression for the first time in the literature. We observed that a single dose of zoledronic acid did not change SDF-1 expression in lung and liver of rats.