Molecularly imprinted electrochemical biosensor for thrombin detection by comparing different monomers
Bioanalysis, cilt.16, sa.6, ss.331-345, 2024 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Derleme
- Cilt numarası: 16 Sayı: 6
- Basım Tarihi: 2024
- Doi Numarası: 10.4155/bio-2023-0203
- Dergi Adı: Bioanalysis
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, EMBASE, MEDLINE
- Sayfa Sayıları: ss.331-345
- Anahtar Kelimeler: biomedical detection, biosensor, molecular imprinted polymers, polymer, thrombin detection
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
Aim: Investigating molecularly imprinted polymers (MIPs) in electrochemical biosensors for thrombin detection, an essential protein biomarker. Comparing different monomers to showcase distinct sensitivity, specificity and stability advantages. Materials & methods: Dopamine, thionine and ethanolamine serve as monomers for MIP synthesis. Electrochemical methods and atomic force microscopy characterize sensor surfaces. Performance is evaluated, emphasizing monomer-specific electrochemical responses. Results: Monomer-specific electrochemical responses highlight dopamine's superior signal change and stability over 30 days. Notably, a low 5 pg/ml limit of detection, a broad linear range (5-200 pg/ml) and enhanced selectivity against interferents are observed. Conclusion: Dopamine-based MIPs show promise for high-performance electrochemical thrombin biosensors, suggesting significant applications in clinical diagnostics.