Investigation of BRAF mutation analysis with different technical platforms in metastatic melanoma


Sener E., Yildirim P., TAN A., GÖKÖZ Ö., Tezel G. G.

PATHOLOGY RESEARCH AND PRACTICE, vol.213, no.5, pp.522-530, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 213 Issue: 5
  • Publication Date: 2017
  • Doi Number: 10.1016/j.prp.2017.01.010
  • Title of Journal : PATHOLOGY RESEARCH AND PRACTICE
  • Page Numbers: pp.522-530

Abstract

In metastatic melanoma, the detection of somatic mutations in the BRAF gene is crucial regarding patient selection for targeted therapy. Several screening methods have been developed to identify BRAF gene mutations. In this study, our objective was to evaluate the detection of the BRAF V600 mutations using two molecular methods, real-time polymerase chain (real-time PCR) assay and pyrosequencing, and immunohistochemistry (IHC), and to compare the results of these different technical platforms.