Investigation of BRAF mutation analysis with different technical platforms in metastatic melanoma


Sener E., Yildirim P., TAN A., GÖKÖZ Ö., Tezel G. G.

PATHOLOGY RESEARCH AND PRACTICE, cilt.213, ss.522-530, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 213 Konu: 5
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.prp.2017.01.010
  • Dergi Adı: PATHOLOGY RESEARCH AND PRACTICE
  • Sayfa Sayıları: ss.522-530

Özet

In metastatic melanoma, the detection of somatic mutations in the BRAF gene is crucial regarding patient selection for targeted therapy. Several screening methods have been developed to identify BRAF gene mutations. In this study, our objective was to evaluate the detection of the BRAF V600 mutations using two molecular methods, real-time polymerase chain (real-time PCR) assay and pyrosequencing, and immunohistochemistry (IHC), and to compare the results of these different technical platforms.