Elevated estradıol ın a prepubertal female: wıthın-laboratory systematıc and practıcal ınterference screenıng
Scandinavian Journal of Clinical and Laboratory Investigation, cilt.85, sa.1, ss.28-33, 2025 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 85 Sayı: 1
- Basım Tarihi: 2025
- Doi Numarası: 10.1080/00365513.2025.2457113
- Dergi Adı: Scandinavian Journal of Clinical and Laboratory Investigation
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, CINAHL
- Sayfa Sayıları: ss.28-33
- Anahtar Kelimeler: endocrinology, Estradiol, ımmunoassay, Liquid Chromatography-Mass Spectrometry, precocious puberty
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
Analytical errors related to endogenous or exogenous substances are a cause of unnecessary investigation, intervention, and patient concern especially in immunoassay platforms. In this report, we systematically screened for estradiol interference using a practical algorithm. For extended research in interference screening, repeated estradiol measurements for control and case samples were carried out for method comparison (three immunoassay platforms and one liquid chromatography-mass spectrometry (LC-MS/MS) measurement), dilution test, polyethylene glycol (PEG) precipitation, and heterophile antibody blocking tube. When serial dilutions were applied to the sample, a deviation from linearity was observed when compared to the control sample. With PEG precipitation, the recovery rate of 63%. While the patient’s serum estradiol value was 389.02 pmol/L using the Siemens Atellica immunoassay method, the same sample was <36.7 pmol/L in the Abbott immunoassay method and 34.9 pmol/L in the Roche immunoassay platform. Applying the LC-MS/MS method, the patient had a serum estradiol of 3.33 pmol/L. Immunoassays are susceptible to interferences that can cause high estimates due to cross-reactivity. Therefore, LC-MS/MS may be more suitable than immunoassays for preventing misdiagnosis and inappropriate treatments, especially for children.