Evaluation of H-800/FUS-100 automatic urine analyzer performance

Ercan M., Oguz E. F., Kaya O., YILMAZ F. M.

Turkish Journal of Biochemistry, cilt.43, sa.1, ss.89-92, 2018 (SCI-Expanded, Scopus, TRDizin)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1515/tjb-2016-0248
  • Dergi Adı: Turkish Journal of Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.89-92
  • Anahtar Kelimeler: Erythrocytes, FUS-100, H-800, Interference, Leukocytes, Urine microscopy
  • Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet

Özet

© 2018 Turkish Biochemistry Society. All rights reserved.Objective: Automated urine analysis is usually preferred for laboratories with intensive workload. The aim of this study was to evaluate the performance of the automated urine analyser H-800/FUS-100 and detect the error sources. Materials and methods: One thousand four hundred fifty nine fresh urine samples were analyzed with H-800/ FUS-100 automated systems. The urine sediment of the samples with discrepant strip and microscopy results were confirmed by manual microscopy. Precision and carry over studies were performed. Results: The discrepancy is detected in a ratio of 5.89% between chemical analysis (H-800) and microscopic analysis (FUS-100) of the device. A total of 86 discrepant samples were detected. Fifty six of 86 were erythrocyte discrepancies (65.1%) and 30 of 86 were leukocyte discrepancies (34.9%). The results of carry over analysis for erythrocyte and leukocyte were 21.85% and 13.64%, respectively. Conclusions: Sixteen (1.09%) of 1459 patients' results in FUS-100 were discrepant with manual microscopy. Commonly, yeasts and crystals affected erythrocyte counts and calcium oxalate and amorphous crystals affected the leukocyte counts. Images should be reviewed for every sample when automated systems are used for urine analysis. Especially if discrepancy is detected between chemical and microscobic analysis, the results should also be confirmed with manual microscopy.