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33rd International Symposium on Pharmaceutical and Biomedical Analysis, Ankara, Turkey, 2 - 06 July 2023, pp.94-95, (Summary Text)
Tyrosinase (Tyr, EC 1.14.18.1), which catalyzes the biosynthesis of melanin and other pigments through oxidation from tyrosine, is a copper-containing metalloenzyme found in the plant bacterial, mammalian, and fungal tissues. In this study, Tyr inhibition was followed with Rosmaniric acid with the selected Rosmaniric acid-containing plants. Firstly, an electrochemical biosensor was developed by immobilizing Tyrosinase in a novel three component organoselenium-based random conjugated polymer matric with NH2 functionalized quantum dots. The response of Tyrosinase towards its well-known substrate, catechol, was optimized in terms of NH2 functionalized quantum dots amount, conducting polymer amount, tyrosinase amount, and glutaraldehyde percent. Following then, Tyrosinase inhibition was observed using Rosmaniric acid-containing plants, Rosmarinus and Eryngium campestre.