Electrochemical Quantification of Parathyroid Hormone Using an Aptamer-Functionalized Magnetic Nanoparticle System
Applied Biochemistry and Biotechnology, cilt.198, sa.11, ss.8864-8883, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 198 Sayı: 11
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s12010-026-05862-z
- Dergi Adı: Applied Biochemistry and Biotechnology
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Compendex, EMBASE, MEDLINE, Natural Science Collection (ProQuest), Biological Science Database (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
- Sayfa Sayıları: ss.8864-8883
- Anahtar Kelimeler: Aptamer, Clinical Validation, Electrochemical Biosensor, Intraoperative Parathormone, Magnetic Nanoparticles, Parathyroidectomy, PTH
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
In this study, we developed a highly sensitive and specific aptamer-based electrochemical biosensor for the quantitative analysis of parathyroid hormone (PTH) for intraoperative parathormone (ioPTH) analysis. By leveraging the intrinsic electrochemical activity of PTH, a direct detection method was first optimized on carbon screen-printed electrodes (SPEs). The biosensor’s performance was then significantly enhanced by using aptamer-functionalized magnetic nanoparticles, which selectively captured and enriched the PTH target from complex matrices. The developed method demonstrated a low limit of detection (LOD) of 1.9 pg/mL and a wide linear range of 5-400 pg/mL, validating its high sensitivity and broad applicability for clinical use. The biosensor also exhibited high specificity against common interferents in biological fluids and excellent long-term stability, retaining over 75% of its initial activity for 3 months. Clinical validation using real patient serum samples showed a high correlation of up to 96.72% with a commercial ELISA kit, confirming the biosensor’s reliability as a rapid, accurate diagnostic tool. The successful integration of aptamer technology with a practical electrochemical platform offers a promising alternative to conventional immunoassays for real-time, point-of-care PTH monitoring.