Performance of different screening methods for the determination of urinary glycosaminoclycans

Afyoncu E., Yilmaz G., Yilmaz F. M., YÜCEL D.

Clinical Chemistry and Laboratory Medicine, vol.51, no.2, pp.347-350, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 51 Issue: 2
  • Publication Date: 2013
  • Doi Number: 10.1515/cclm-2012-0471
  • Journal Name: Clinical Chemistry and Laboratory Medicine
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.347-350
  • Keywords: extracellular matrix, glycosaminoglycans, method comparison, proteoglycans, urine
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

Background: The study aim was to compare the performance of three different methods used for determining urinary glycosaminoglycans (GAG) levels in spot and 24-h urine samples. Methods: Performance characteristics were studied for cetylpyridinium chloride (CPC), and manual and automated dimethylmethylene blue (DMB) methods. Results: For automated DMB method, within-run precisions were 9.10% and 1.98%, and between-day precisions were 13.0% and 5.81% in low- and high-urine pools, respectively. The method was linear up to 100 mg/L of GAG concentration. The detection limit of the method was 0.71 mg/L. Mean recovery was 95.7%. Conclusions: The automated DMB method was found to give better performance characteristics than cetylpyridinium chloride (CPC) and manual DMB methods. It is a fast, cheap, simple and reliable method and can be applied in many diseases in which GAG is used as a screening test. © 2013 by Walter de Gruyter Berlin Boston.