© 2020 Wiley Periodicals LLCBackground: Androgenetic alopecia (AGA) is the most common type of hair loss and affects approximately 50% of the male population. Aims: In the present study, to investigate microinflammation, perifollicular fibrosis, and oxidative stress in AGA cases, some serum biomarker levels were measured and evaluated. Patients/Methods: Serum samples were drawn from patients (n = 58) and control (n = 30) groups referring to Atatürk Training and Investigation Hospital Dermatology Outpatient clinic. In serum samples, NF-κB, TNF-α, TGF-β1, thioredoxin, nitric oxide, TOS, TAS, and thiol disulfide homeostasis (native thiol, total thiol, disulfide) were measured and evaluated. Results: In patients with AGA, NF-κB (P =.005), TNF-α (P =.008), TGF-β1 (P =.028), thioredoxin (P =.004), nitric oxide (P <.001), and TOS (P <.001) serum levels were found to be significantly higher than those in control group, while TAS (P =.003), native thiol (P <.001), total thiol (P <.001), and disulfide (P <.001) serum levels were found to be significantly lower. Conclusions: According to the results of the present study, it was concluded that in that AGA androgens lead to oxidative stress by increasing free oxygen radicals, which accelerates hair loss by causing microinflammation and fibrosis. The recognition of the effect of androgens and associated factors on the hair follicle cycle is essential for the development of new and effective treatment methods.