Thiol/disulfide homeostasis and oxidant status in children with congenital heart disease Doğumsal kalp hastalığı olan çocukların tiyol/disülfid dengesi ve oksidatif durumu

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Sogut I., KAR F., Senat A., Duymaz T., EREL Ö., Salihoglu E.

Turkish Journal of Biochemistry, vol.47, no.3, pp.257-265, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.1515/tjb-2021-0172
  • Journal Name: Turkish Journal of Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.257-265
  • Keywords: ceruloplasmin, congenital heart surgery, disulfide, ischemia modified albumin, myeloperoxidase (MPO), oxidative stress, prolidase, thiol, disulfid, Dogussal kalp cerrasi, iskemik modifiye albumin, miyeloperoksidaz, oksidatif stres, prolidaz, seruloplazmin, tiyol
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

© 2021 Ibrahim Sogut et al., published by De Gruyter.Objectives: This article aims to explain the altered oxidative status and thiol/disulfide homeostasis before and after surgery in children with congenital heart disease (CHD). Methods: Blood samples were taken from the patients (n=50) before the operation (baseline), at the 1st hour, and at the 24th hour after the operation. Thiol-disulfide levels, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), myeloperoxidase (MPO), ceruloplasmin, albumin, ischemia-modified albumin (IMA), and prolidase activities of all samples were measured. Preoperative oxygen saturation (SaO2) values and neutrophil/ lymphocyte ratios (NLR) were also measured. Results: Before the operation, TOS, OSI, MPO, ceruloplasmin, IMA, NRL, and disulfide levels were higher in the cyanotic group than in the acyanotic group. When the indicated three different time points were compared, the TOS, OSI levels, and MPO activities of the 24th hour were significantly lower than the baseline and 1st hour. In comparison, ceruloplasmin levels of 1st hour were significantly higher than of the baseline and 24th hour. Native thiol and total thiol levels in the baseline group were significantly lower than in the 1st and 24th hours. Disulfide levels of the 24th hour were significantly lower than of the baseline. Conclusions: The operation leads to changes in the thiol-disulfide balance and oxidant status in CHD.