Determination of Circulating Tumor Cells by Flow Cytometry in the Bladder Cancer Patients

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ERCAN E., ŞİMŞEK E., ÖZENSOY GÜLER Ö., CANDA A. E., ATMACA A. F., ÇARHAN A.

Urology and Andrology, vol.2, no.1, pp.25-30, 2018 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 2 Issue: 1
  • Publication Date: 2018
  • Doi Number: 10.17140/uaoj-2-113
  • Journal Name: Urology and Andrology
  • Page Numbers: pp.25-30
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

Background

The current study aimed to detect of circulating tumor cells (CTCs) in 7.5 mL blood sample in bladder cancer patients treated with

transurethral resection (TUR) or radical cystectomy (RC) and compare the pre-operative and post-operative CTC counts.

Materials and Methods

CTCs were detected in peripheral blood of 10 bladder cancer patients with flow cytometry using biomarkers EpCAM, CK 14, 15, 16,

19 and CK 7, 8 for positive selection and CD45 for negative selection. The blood samples obtained before and after operation were

studied in 5 out of 10 patients.

Results

The CTC counts decreased significantly in patients (Z=2.032; p=0.042) after the operation. The pre-operative CTC counts for the

patient group were found to be significantly higher than the control group. No CTCs were detected in the control group.

Conclusion

The results are promising in terms of CTC detection in bladder cancer (Bca) by flow cytometry. Pre- and post-operative CTC counts

as predictive and prognostic biomarker dramatically change in this study.

Keywords

Bladder cancer (Bca); Circulating tumor cells (CTCs); Flow cytometry.