It can be said that the concept of gender perceptions, which is expressed as some meanings (behavior, lifestyle and roles, etc.) attributed to genders by societies, has an important place in cultural elements in social life. When it considered, individuals carry the cultural structure of the society they live in a certain ratio, it can be predicted that gender perceptions can also enter into organizations together with employees. From this point of view, it is aimed to examine the concepts of organizational culture and gender perception together. There are a lot of studies which link organizational culture and gender perception with different concepts. This study aims to investigate the possible relationship of organizational culture and its dimensions with gender perception. For this purpose, correlation analyses have been made between gender perception and organizational culture considering each of its sub-dimensions. In this context, organizational culture has been handled within the framework of the Cameron and Quinn Model, and the dimensions of this model, namely clan culture, adhocracy culture, hierarchy culture and market culture, have been subjected to hypotheses. The population of the research is composed of public employees in Ankara. The questionnaire form belonging to the research was delivered to the participants online using the snowball method. The number of people reached is 410, and 386 of them have been observed to be suitable for evaluation. According to the findings of the analyses; organizational culture and its sub- dimensions, clan, hierarchy and market cultures have been concluded that there is a significant relationship with gender perception. On the other hand, it has been found that the adhocracy culture dimension has no relationship with the gender perception variable. It may be suggested to conduct the research with different groups on the basis of different provinces.