Investigation of heavy metal effects on immobilized paraoxanase by glutaraldehyde.
Journal of enzyme inhibition and medicinal chemistry, cilt.28, sa.3, ss.440-6, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 3
- Basım Tarihi: 2013
- Doi Numarası: 10.3109/14756366.2011.647007
- Dergi Adı: Journal of enzyme inhibition and medicinal chemistry
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.440-6
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Hayır
Özet
Serum paraoxonase 1 (PON1) was purified from bovine serum using hydrophobic interaction chromotography on Sepharose 4B-coupled L-tyrosine 1-naphthylamine gel, and monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Paraoxonase enzyme was immobilized using different ratios of glutaraldehyde and the maximum activity was observed with 7% glutaraldehyde. The effects of inhibition by Mn+2, Co+2 and Cu+2 heavy metals on the immobilized and free enzyme activities were studied. At the optimum pH and temperature, the K-m and V-max kinetic values for bovine serum paraoxonase and immobilized paraoxonase towards paraoxon substrate were determined as 0.296 x 10(-3) M & 37.04 EU vs. 0.727-10(-3) M & 36.36 EU, respectively.