© 2020 Wiley Periodicals LLCThis study was conducted to evaluate the effects of ethylenediaminetetraacetic acid (EDTA), Irritrol, and QMix solutions used with manual irrigation and diode and erbium, chromium-doped yttrium, scandium, gallium, and garnet (Er,Cr:YSGG) laser activation on the bonding ability of sealer to the intraradicular dentin. The root canals of 90 mandibular incisors were instrumented, and the teeth were allocated randomly to nine groups (n = 10/group) receiving final irrigation with different irrigation solutions (EDTA, Irritrol, and QMix) and irrigant activation techniques (manual, diode laser, and Er,Cr:YSGG laser). Following endodontic filling, three sections of 1-mm thickness were obtained from the middle third of each root. The root filling was subjected to a push-out test to examine the bond strength of the sealer to dentin. The test data were examined using analysis of variance with a significance level of ɑ <.05. Failure modes were determined by stereomicroscopy, and the surface characteristics of dentin were observed by scanning electron microscopy. Irritrol showed less push-out bond strength than did the other solutions in the Er,Cr:YSGG laser groups (p <.05). In the diode laser groups, QMix provided greater adhesion than did Irritrol (p <.05). The activation of QMix with the Er,Cr:YSGG laser increased the bond strength more than did manual irrigation (p <.05). No other significant difference was observed between other comparison pairs (p >.05). QMix provided adhesion superior to that of Irritrol when activated with diode and Er,Cr:YSGG lasers. Er,Cr:YSGG laser activation increased the adhesion of filling material with QMix irrigation.