Relationship Between the Levels of Holotranscobalamin and Vitamin B12 in Children

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Ok Bozkaya I., Yarali N., Kizilgün M., ÖZKAN S., Tunc B.

Indian Journal of Hematology and Blood Transfusion, vol.33, no.4, pp.537-540, 2017 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.1007/s12288-017-0789-9
  • Journal Name: Indian Journal of Hematology and Blood Transfusion
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.537-540
  • Keywords: Children, Holotranscobalamin, Vitamin B12


© 2017, Indian Society of Haematology & Transfusion Medicine.The purpose of this study was to evaluate the relationship between the plasma holoTC and serum vitamin B12 in children and to identify a cutoff cobalamin values according to holoTC. One hundred and fifty-five children were enrolled into the study. All children were evaluated for hemoglobin, vitamin B12, folate, ferritin and holoTC levels. Children were grouped as with low vitamin B12 level (≤200 pg/mL, group I) and normal vitamin B12 (>200 pg/mL, group II). Serum vitamin B12, and holoTC levels were performed in each patient in the study. In 101 patients with low vitamin B12 (group I) the mean holoTC was 21.74 ± 1.14 pmol/L. In 54 children with normal vitamin B12 (group II) mean holoTC was 44.0 ± 2.7 pmol/L (p < 0.01). A ROC curve analysis was performed to delineate the optimum cut-off point for vitamin B12 level and it was found to be 165 pg/mL with a sensitivity of 70% and specificity of 74%; the area under curve was 0.783 (p < 0.01). Our study displayed a positive correlation between vitamin B12 and holoTC, and defined an optimum cutoff value for vitamin B12 as 165 pg/mL. Further studies using the markers both MMA, tHcy and holoTC to confirm the findings are needed.