Research Information System
Transfusion and Apheresis Science, vol.50, no.3, pp.467-472, 2014 (SCI-Expanded)
© 2014 Elsevier Ltd.The study was designed to compare colony forming capacity of granulocyte-colony stimulating factor (G-CSF) stimulated bone marrow (G-BM) with standard unstimulated bone marrow (U-BM) of healthy donors of pediatric patients.CFU-Assay results of 26 healthy donors of pediatric patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) were analyzed retrospectively. 13 of donors received 10. μg/kg per day of G-CSF as a single injection for 3 consecutive days and other 13 of donors had unstimulated BM. Colony forming capacity of hematopoietic stem cells evaluated with Colony Forming Unit-Assay (CFU-Assay) with in semi-solid agar culture medium after 14-18. days of culture period.CFU-Assay results of G-BM and U-BM (expressed as means) were; Burst Forming Unit-Erythroid (BFU-E): 15.20×104/kg and 8.38×104/kg, Colony Forming Unit-Granulocyte Macrophage (CFU-GM): 10.35×104/kg and 5.67×104/kg, Colony Forming Unit-Erythroid (CFU-E): 0.59×104/kg and 0.33×104/kg, CFU-Granulocyte Erythroid Macrophage Megakaryocyte (CFU-GEMM): 0.52×104/kg and 0.53×104/kg respectively.BFU-E and CFU-GM capacity of G-BM was increased and statistically significantly different than standard U-BM (p≤. 0.01).In conclusion, increased colony forming capacity of hematopoietic stem cells of G-BM when compared with standard unstimulated BM could be a major advantage for transplantation.