© 2020 Egyptian Society of Rheumatic DiseasesAim of the work: Activity limitation is one of the most common causes of disability in people with knee and hip osteoarthritis (OA). The present study aimed to determine the reliability and validity of the Turkish adaptation of Animated Activity Questionnaire (AAQ) in patients with hip and knee OA. Patients and methods: Two hundred knee and hip OA patients aged 18–80 were assessed using the AAQ. Internal consistency and test–retest measurements were performed to determine reliability. A subgroup of 40 patients completed the AAQ again after seven days to determine test–retest reliability. In order to validate Turkish version of the AAQ, all patients completed the AAQ, the Western Ontario and McMaster Universities Arthritis Index (WOMAC), Knee Injury and Osteoarthritis Outcome Score-Physical Function Shortform (KOOS-PS) and Hip Disability and Osteoarthritis Outcome Score-Physical Function Short form (HOOS-PS). Another subgroup of 136 patients performed the timed up and go (TUG) test, the 30-second chair stand test (CST), and the stair climbing test (SCT). Results: The Cronbach alpha value of the AAQ was 0.94, demonstrating high internal consistency. The test–retest score of the AAQ was 0.97, which was also high. The AAQ exhibited negative and moderate correlation with the WOMAC, KOOS-PS and HOOS-PS (0.59, −0.60, and −0.49, respectively). Stronger and high correlations were determined between the AAQ and TUG, CST, SCT tests (-0.74, 0.62, and −0.58, respectively). Conclusion: The study findings suggest that the AAQ is a reliable and valid tool for evaluating activity limitations in Turkish knee and hip OA patients.