Detection of Bartonella spp. in field mice (Microtus socialis) by culture and PCR Tarla farelerinde (Microtus socialis) Bartonella Spp. Varlıǧinın kültür ve PCR yöntemleriyle belirlenmesi

KARAGÖZ A., Çelebi B., ŞİMŞEK H., Taner M., Kiliç S., DURMAZ R., ...More

Ankara Universitesi Veteriner Fakultesi Dergisi, vol.60, no.4, pp.235-239, 2013 (SCI-Expanded, Scopus, TRDizin) identifier

  • Publication Type: Article / Article
  • Volume: 60 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1501/vetfak_0000002585
  • Journal Name: Ankara Universitesi Veteriner Fakultesi Dergisi
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.235-239
  • Keywords: Bartonella, Microtus socialis, PCR
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

The species within the genus Bartonella are intracellular bacteria causing long-lasting bacteremia in humans and animals. Eleven Bartonella species isolated from animals have zoonotic characteristics. The four of zoonotic Bartonella species have been reported in rodents. In this study, Bartonella spp. in 42 field mice, (Microtus socialis), captured from one spot in Central Anatolia Region were investigated by culture and molecular methods. The positivity of Bartonella was 16.6% (7/42) by culture, and 57.1% (24/42) by PCR (Polymerase Chain Reaction) from liver tissues of field mice. The citrate synthesis gene regions (gltA) of the isolated seven strains were identified by DNA sequencing analysis. It was determined that five isolates were Bartonella taylorii, and two isolates were Bartonella grahamii which are zoonotic. This study is the first report for the presence of Bartonella spp. in rodents in Turkey. Tarla farelerinde (Microtus socialis) Bartonella Spp. varhgini{dotless}n kültür ve PCR yöntemleriyle belirlenmesi.