Targeting Ras Signaling via Carbonic Anhydrase IX Inhibition: Differential Impact of Acetazolamide on Clear Cell Renal Carcinoma and Melanoma Cells
EXPERIMED, cilt.15, sa.3, ss.1-8, 2025 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 15 Sayı: 3
- Basım Tarihi: 2025
- Doi Numarası: 10.26650/experimed.1703562
- Dergi Adı: EXPERIMED
- Derginin Tarandığı İndeksler: Scopus, Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.1-8
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
Objective: To investigate the effect of carbonic anhydrase IX (CA-IX) inhibition by acetazolamide (AAZ) on
Ras protein expression in clear cell renal carcinoma and melanoma cell lines.
Materials and Methods: CAKI-2 (clear cell renal carcinoma) and A375 (melanoma) cell lines were cultured
and treated with AAZ, rapamycin (RAPA), or vemurafenib (VMF). Cytotoxicity was assessed using the WST-1
assay. CA-IX inhibition was confirmed by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence staining. Ras protein expression levels were determined by Western blot analysis.
Results: In CAKI-2 cells, there was no significant change in Ras protein expression following AAZ or
RAPA treatment (p>0.05). In A375 melanoma cells, AAZ treatment significantly reduced Ras protein levels
compared with both the untreated control and VMF-treated groups (p<0.001).
Conclusion: AAZ significantly reduced Ras protein expression in A375 melanoma cells. These findings
provide evidence supporting the role of AAZ in regulating Ras-MAPK signaling in melanoma.