The attraction of paraoxonase-1 associated with the MAPK pathway on colon carcinoma cells
Turkish Journal of Biochemistry, vol.49, no.1, pp.110-115, 2024 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 49 Issue: 1
- Publication Date: 2024
- Doi Number: 10.1515/tjb-2023-0072
- Journal Name: Turkish Journal of Biochemistry
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Food Science & Technology Abstracts, Directory of Open Access Journals
- Page Numbers: pp.110-115
- Keywords: antioxidant, colon cancer, ERK pathway, oxidative stress, paraoxonase-1
- Open Archive Collection: AVESIS Open Access Collection
- Ankara Yıldırım Beyazıt University Affiliated: Yes
Abstract
Objectives: A comprehensive understanding of the role of PON enzymatic activities may play an important role in the etiology and prevention of many cancers. PON1 is known as a potent antioxidant that scavenges free radicals in the human body. The enzymatic activities of paraoxonase (PON1) and mitogen-activated protein kinase (MAPK) signalling pathways in colorectal cancer are being investigated to determine whether they hold promise for novel diagnostic or therapeutic applications in colorectal cancer. Methods: HT-29 colon cancer cell lines and CCD-18Co colon cell lines were used. B-Raf, p-B-Raf, ERK, and p-ERK proteins involved in MAPK signalling pathways and serum levels of PON1 were detected and analyzed by the Western blotting method. Results: The levels and activity of PON1 enzyme were significantly decreased in HT-29 cells compared to CCD-18Co cells (p=0.0173 and p=0.0281, respectively). The levels of p-B-Raf and p-ERK, which activates the MAPK signalling pathway, were significantly increased in HT-29 cells (p=0.0037 and p=0.0074, respectively). Conclusions: A positive correlation was found between PON1 level and PON1 enzyme activity (p=0.008). A negative correlation was found between PON1 level and p-B-Raf/p-ERK protein levels, which play a role in the MAPK signalling pathway (p=0.016 and p=0.036, respectively). A negative correlation was also found between PON1 enzyme activity and p-B-Raf/p-ERK protein levels (p=0.060 and p=0.037, respectively). It is suggested that the increase of proteins involved in the MAPK signaling pathway in cancer cells is caused by the decreasing serum levels of PON1 and enzymatic activity of PON1.