Antimicrobial activity evaluations of four different types of urethral catheters

Aslan B., Şerefoğlu E. C. , Çırak T., Açıkgöz Z. , Denkbaş E. B.

European Urology Supplements, cilt.8, ss.228, 2009 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 8 Konu: 4
  • Basım Tarihi: 2009
  • Dergi Adı: European Urology Supplements
  • Sayfa Sayıları: ss.228

Özet

Antimicrobial activity evaluations of four different

types of urethral catheters

Aslan B.1, Serefoglu E.C.2, Cirak T.1, Acikgoz Z.C.3, Denkbas E.B.1

1Hacettepe University, Division of Nanotechnology and Nanomedicine, Ankara, Turkey, 2Ataturk

Training and Research Hospital, Dept. of 1st Urology, Ankara, Turkey, 3Ataturk Training and

Research Hospital, Dept. of Clinical Microbiology, Ankara, Turkey

Introduction & Objectives: To evaluate the antimicrobial activity of four different types of

commercially available urethral catheters and determine their advantages in patients with urinary

tract infections.

Material & Methods: Four different types of 20 Fr commercially available urethral catheters

(Silicone, Silicolatex, Nitrofurazone coated, Silver nitrate coated) were cut into 1 cm pieces. Ten

catheter pieces from each type were incubated in physiological buffered saline including either

slime positive Staphylococcus aureus or slime negative Escherichia coli with 0.5 McFarland

turbidity. After 24 and 48 hours, 5 catheter pieces were washed with saline and vortexed for 2 min

in 1.5 ml of physiological saline. There were 100 μl of samples of vortexed material inoculated on

blood agar and colony numbers were assessed. Additionally, in vitro drug release of nitrofurazone

coated catheter was assessed in physiological buffer solution with pH: 7.4 at 37ºC.

Results: The mean colony numbers of Staphylococcus aureus at 24 hour were 850, 4200, 350

and 3400 for Silicone, Silicolatex, Nitrofurazone coated, Silver nitrate coated catheter pieces

respectively, whereas the mean colony numbers of Escherichia coli at 24 hour were 78500,

95000, 34000 and 35850 for them. At 48 hour, the mean colony numbers of Staphylococcus

aureus were 33350, 33350, 36700 and 41700; And the mean numbers of Escherichia coli were

100000, 96700, 31750 and 46700 for Silicone, Silicolatex, Nitrofurazone coated, Silver nitrate

coated catheter pieces, respectively (table 1). In the in vitro drug release studies of nitrofurazone

coated catheter it was found that almost all of nitrofurazone was released within the first 24 hours,

and this finding was correlated with the bacterial adherence results of those catheters.

Table 1. The mean colony numbers of S. Aureus and E. Coli at 24 and 48 hours