Development of a novel UPLC approach to co-prediction of four active compounds in a multi-component pharmaceutical preparation
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol.1240, 2024 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 1240
- Publication Date: 2024
- Doi Number: 10.1016/j.jchromb.2024.124159
- Journal Name: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
- Keywords: Chromatographic separation, Quaternary mixture, Simultaneous determination, UPLC-PDA
- Ankara Yıldırım Beyazıt University Affiliated: Yes
Abstract
In this work, a new ultra-performance liquid chromatography method based on photodiode array detection (UPLC-PDA) was first developed for the quantitative analysis of the quaternary mixture of ascorbic acid (AA), paracetamol (PAR), caffeine (CAF) and chlorpheniramine maleate (CPA) in a commercial dosage form. The developed UPLC-PDA method offered a new possibility for the co-determination of four active ingredients in a drug combination with short run time and simple sample preparation. The successful chromatographic separation of the four drugs was performed using a Waters Acquity UPLC BEH C18 column (1.7 µm 2.1 × 100 mm) (Mildford, USA) and a mobile phase consisting of water (12 %), acetonitrile (13 %) and 0.1 M H3PO4 (75 %) at a flow rate of 0.25 mL/min. The validation of the proposed UPLC-PDA approach was verified by analyzing synthetic mixtures, inter- and intra-day experiments, and commercial powder samples and provided satisfactory results.