Protective effects of Silymarin on testicular torsion/detorsion in rats
European Review for Medical and Pharmacological Sciences, vol.27, no.21, pp.10446-10453, 2023 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 27 Issue: 21
- Publication Date: 2023
- Doi Number: 10.26355/eurrev_202311_34319
- Journal Name: European Review for Medical and Pharmacological Sciences
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, MEDLINE, Directory of Open Access Journals
- Page Numbers: pp.10446-10453
- Keywords: Ischemia-reperfusion, Silymarin, Spermatogenesis, Testicular damage, Testicular torsion
- Ankara Yıldırım Beyazıt University Affiliated: Yes
Abstract
OBJECTIVE: The present research aimed to study the possible protective effects of Silymarin on testicular I/R injury in a rat model evaluated through histopathology and biochemical parameters. MATERIALS AND METHODS: This research investigated the impact of Silymarin on IR damage in male Wistar albino rats. Animals were divided into three groups: group 1 (sham), group 2 (IR), and group 3 (IR+Silymarin). RESULTS: There were no notable differences in the levels of malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) across the three groups (p=0.260, p=0.486 and p=0.803, respectively). Contrarily, the total antioxidant status (TAS) levels exhibited significant variations between groups (p=0.001). The total oxidant status (TOS) levels also differed significantly between groups (p=0.004). The tissue evaluations uncovered substantial differences in the Johnson score, which is used to gauge testicular damage. A distinct contrast was seen between Group 1 and Group 2, and also between Group 2 and Group 3, with an all-encompassing p-value lower than 0.01. The same significant disparities were found for the percentages of Bax and Annexin V immunostaining (p<0.01 for each), reflecting the inflammation and apoptosis brought about by ischemia-reperfusion and the protective effects of the treatment. CONCLUSIONS: The outcomes of the current investigation showed that Silymarin could be a valuable agent for reducing testicular tissue damage following I/R injury.