The effects of pregabalin on kidney tissue of the rats those have ureter obstruction Üreter Obstrüksiyonu yapılan ratlarda pregabalinin Böbrek Dokusu üzerine etkileri


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Yilmaz Z., ARPACI A. H., SÜNGÜ N., KILIÇARSLAN A., ŞIVGIN V., İNAN N., ...More

Gazi Medical Journal, vol.30, no.1, pp.55-59, 2019 (Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 1
  • Publication Date: 2019
  • Doi Number: 10.12996/gmj.2019.14
  • Journal Name: Gazi Medical Journal
  • Journal Indexes: Scopus
  • Page Numbers: pp.55-59
  • Keywords: Caspase 3, Caspase 8, MDA, NO, Pregabalin, Ureter obstruction
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

© Copyright 2019 by Gazi University Medical Faculty.Background: Various algorithms and treatment guidelines have been established for the treatment of neuropathic pain. In summary, tricyclic antidepressants, anticonvulsants, serotonin reuptake inhibitors and opioids are used in these algorithms. Pregabalin is an anticonvulsant drug that is frequently used in the treatment of neuropathic pain. During the use of kidneys in patients with renal insufficiency due to the need to change the dose was taken attention. Therefore, in our study, we aimed to investigate the effects of pregabalin on renal tissue in rats with renal failure due to ureter obstruction. Materials and Methods: After the approval of the ethics committee, 24 rats were randomly divided into four groups: Group C: Control, Group P: Pregabalin, Group UO: Ureter Obstruction, UO-P Group: Ureter Obstruction - Pregabalin. Urinary obstruction groups underwent a low abdominal incision under ketamine anesthesia to reach the distal right ureter and suture was placed with 2.0 mersilene and was waited for 3 weeks for late stage renal failure. In group P and UO-P rats, pregabalin was administered intraperitoneally at a dose of 100 mg / kg. After the twenty-four hour followup period, rats were sacrificed with the blood taken from the intraabdominal aorta by intraperitoneal ketamine (100 mg / kg). Blood samples were stored at -20 ° C and urea, creatinine, MDA and NO were studied. In the kidney tissue, caspase 3, 8 enzymes and histopathological evaluation were performed. Results: Caspase 3 enzyme activity was significantly increased in UO groups. In addition, pregabalin administration significantly increased the activity of caspase 3 in the UO-P group compared to the P group. The enzyme activity of caspase 8 was similar between the groups. Light microscopy revealed significant changes in UO groups, especially in the kidney tissue, which was obstructed. NO and MDA enzyme activities increased significantly in UO groups. In addition, pregabalin administration significantly increased NO and MDA enzyme activities in UO-P group compared to P group. Urinary obstruction significantly increased urea and creatinine levels. Pregabalin administration also increased urea and creatinine levels in the UO-P group compared to the P group. Conclusion: Pregabalin, which is frequently used in the treatment of neuropathic pain, has not been found to increase the renal damage that occurs experimentally in renal failure rats. We believe that our findings should be supported by a wide range of experimental and clinical studies.