Histopathological Examination of the Effect of Pioglitazone on the Rat Spleen with Metabolic Syndrome
6 th International Conference on ADVANCES IN NATURAL and APPLIED SCIENCES, Ağrı, Türkiye, 11 - 13 Ekim 2022, ss.354-355, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Ağrı
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.354-355
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
The metabolic syndrome (MS) is linked to a wide range of metabolic conditions, particularly type 2 diabetes mellitus, central obesity, insulin resistance, hypertension, dislipidemia, and cardiovascular diseases (T2DM). Numerous organs, including the liver, heart, spleen, pancreas, and adipose tissue, are damaged by MS [1,2,3]. Pioglitazone (PGZ), a TDZ that belongs to the class of anti-diabetic drugs, lowers blood glucose levels in T2DM patients' peripheral circulation. PGZ is used either alone or in combination with another drug's active ingredient, such as metformin, insulin, or another [4]. The pathophysiology and mechanisms of MS are still being researched, though. We aimed to investigate the effect of pioglitazone (PGZ) on the spleen of rats with MS. Male Wistar rats were divided into three groups, such as the control group, MS, and MS+PGZ groups. Metabolic syndrome was induced by providing drinking water that was 32% sucrose for 18 weeks. In order to detect the effect of pioglitazone, rats were given intragastric PGZ for 8 weeks. All of the animals were exposed to a 12 h light–12 h dark cycle. Abdominal obesity and glucose intolerance have been measured as markers of metabolic syndrome. At the end of the treatment period, animals from each group were sacrificed and embedded in paraffin for histopathological analysis. The MS rats had altered body weight, abdominal obesity, and glucose intolerance, indicating the successful establishment of our MS model. Histopathological analysis was evaluated by ImageJ and was used by GraphPad Prism statistically. The typical rat spleen had the normal arrangement of red pulp and white pulp structures. The number of follicles, marginal zone area, fibrosis, and red pulp size area were significantly increased in MS rats and decreased in MS+PGZ rats. In future studies, the detailed effect of pioglitazone can be supported by histochemical, immunological, and molecular biological studies.