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Archives of Gynecology and Obstetrics, vol.305, no.3, pp.617-623, 2022 (SCI-Expanded, Scopus)
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.Purpose: This study aimed to determine the potential clinical use of dynamic thiol–disulfide balance in cases with preinvasive lesions of the cervix. Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, 100 patients with low-grade squamous intraepithelial lesion, and 110 healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol–disulfide parameters. The ischemia-modified albumin, total oxidant–antioxidant capacity, and oxidative stress index of the retrieved cases were further analyzed. Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p: 0.004 and 0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p: 0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p: 0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p: 0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r: 0.420, p < 0.001). Conclusion: The analysis of dynamic thiol–disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus-related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol–disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.