In Vitro evaluation of thymoquinone on apoptosis and oxidative DNA damage in high glucose condition


Gümüş A. F. , Dede S., Yüksek V., Çetin S., Taşpınar M.

Cellular and Molecular Biology, vol.64, no.14, pp.79-83, 2018 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 14
  • Publication Date: 2018
  • Doi Number: 10.14715/cmb/2018.64.14.13
  • Journal Name: Cellular and Molecular Biology
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.79-83
  • Keywords: Caspases, DNA damage, High glucose, In vitro, Thymoquinone

Abstract

© 2018 by the C.M.B. Association. All rights reserved.The study was planned to investigate the effects of thymoquinone (TQ), which is a compound in N. sativa, on caspase dependent apoptosis and oxidative DNA damage in high glucose treated PC12 cells. PC12 cells were treated with high glucose (G1-150 mM, G2-250 mM, G3-350 mM), TQ (20 µM), and their combinations. Oxidative DNA damage (8-OHdG (8-Oxo-2'-deoxyguanosine)), and apoptosis (caspase 3, caspase 8, caspase 9 enzymes and M30 protein) parameters were analyzed with ELISA. The 8-OHdG levels decreased in all combination groups compared to the control (p≤0.001). There was no statistically significant difference between caspase 3 and 9. Caspase 8 in TQ, G3, TQG1, TQG2 groups were higher than the control (p≤0.002). Low M30 levels were observed in TQG1 group (p≤0.002). In conclusion, it was observed that in PC12 cell line treated with the high glucose concentrations, TQ administration had a statistically significant effect on oxidative DNA damage and some apoptotic parameters (caspase 8 and M30 protein).