In Vitro evaluation of thymoquinone on apoptosis and oxidative DNA damage in high glucose condition

Gümüş A. F., Dede S., Yüksek V., Çetin S., Taşpınar M.

Cellular and Molecular Biology, vol.64, no.14, pp.79-83, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64 Issue: 14
  • Publication Date: 2018
  • Doi Number: 10.14715/cmb/2018.64.14.13
  • Journal Name: Cellular and Molecular Biology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.79-83
  • Keywords: Caspases, DNA damage, High glucose, In vitro, Thymoquinone
  • Ankara Yıldırım Beyazıt University Affiliated: No

Abstract

© 2018 by the C.M.B. Association. All rights reserved.The study was planned to investigate the effects of thymoquinone (TQ), which is a compound in N. sativa, on caspase dependent apoptosis and oxidative DNA damage in high glucose treated PC12 cells. PC12 cells were treated with high glucose (G1-150 mM, G2-250 mM, G3-350 mM), TQ (20 µM), and their combinations. Oxidative DNA damage (8-OHdG (8-Oxo-2'-deoxyguanosine)), and apoptosis (caspase 3, caspase 8, caspase 9 enzymes and M30 protein) parameters were analyzed with ELISA. The 8-OHdG levels decreased in all combination groups compared to the control (p≤0.001). There was no statistically significant difference between caspase 3 and 9. Caspase 8 in TQ, G3, TQG1, TQG2 groups were higher than the control (p≤0.002). Low M30 levels were observed in TQG1 group (p≤0.002). In conclusion, it was observed that in PC12 cell line treated with the high glucose concentrations, TQ administration had a statistically significant effect on oxidative DNA damage and some apoptotic parameters (caspase 8 and M30 protein).