Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.Objective Dynamic thiol-disulphide homeostasis is an indicator of the antioxidant system that interacts early with the oxidative environment. This study aimed to assess the dynamic thiol/disulphide balance in individuals with white-coat hypertension (WCH). Methods This cross-sectional study included a total of 117 individuals who presented to our outpatient cardiology clinic. Seventy patients were diagnosed with WCH. All blood samples were obtained from the patients after a minimum of 8h of fasting, centrifuged immediately, stored in Eppendorf tubes, and protected at −80°C. Results The two groups were well-balanced in terms of age, gender, and BMI. Native thiol and total thiol levels were lower in the WCH group than in normotensive individuals. However, disulphide levels were higher in the former. Serum disulphide concentration positively correlated with 24-h SBP, and 24-h DBP. In backward stepwise logistic regression analysis, serum disulphide [odds ratio (OR)=1.165 (1.089-1.245), 95% confidence interval (CI), P<0.001] and serum total thiol levels [OR=0.992 (0.984-1.000), 95% CI, P=0.050] were independently associated with WCH. Conclusion We demonstrated that dynamic thiol/disulphide balance shifted strongly towards disulphide formation due to thiol oxidation in patients with WCH.