Phytochemicals and Biofunctional Properties of Portulaca oleracea L.: Integrated in Vitro and in Silico Analyses
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, cilt.69, sa.e26250832, ss.1-24, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 69 Sayı: e26250832
- Basım Tarihi: 2026
- Doi Numarası: 10.1590/1678-4324-2026250832
- Dergi Adı: BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
- Derginin Tarandığı İndeksler: Scopus, Science Citation Index Expanded (SCI-EXPANDED), BIOSIS, Directory of Open Access Journals
- Sayfa Sayıları: ss.1-24
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
Portulaca oleracea L. (purslane) is a nutritionally and pharmacologically important
medicinal plant traditionally valued for its therapeutic benefits. This study
aimed to comprehensively investigate the phytochemical composition,
antioxidant, and anti-inflammatory activities of its aqueous extract, supported
by in silico analyses. Total phenolic, flavonoid, alkaloid, and tannin contents
were quantified as 9.85 mg GAE/g, 4.42 mg naringenin/g, 0.61 mg caffeine/g, and
0.05 mg epicatechin/g, respectively. HPLC profiling identified ferulic acid
(268.33 µg/g), allantoin (140.66 µg/g), and oleracein A (321.98 µg/g) as major
bioactive constituents. In vitro antioxidant assays (DPPH, ABTS, and FRAP) demonstrated
strong radical scavenging and reducing power, while protein denaturation and
erythrocyte membrane stabilization tests confirmed significant
anti-inflammatory activity. In silico molecular docking analyses revealed high
binding affinities of these compounds to inflammation and Helicobacter
pylori protein targets, suggesting potential anti-inflammatory and
antimicrobial mechanisms at the molecular level. These findings indicate that
purslane is a rich source of bioactive molecules capable of exerting potent
antioxidant and anti-inflammatory effects. The integration of phytochemical, in
vitro, and in silico approaches provides strong scientific evidence supporting
its therapeutic potential.