CARBON NANOTUBES MODIFIED ELISA SYSTEM DEVELOPMENT FOR IMPROVED DETECTION OF BIOMARKERS
Mugla Journal of Science and Technology, cilt.9, sa.1, ss.80-86, 2023 (TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 9 Sayı: 1
- Basım Tarihi: 2023
- Doi Numarası: 10.22531/muglajsci.1261541
- Dergi Adı: Mugla Journal of Science and Technology
- Derginin Tarandığı İndeksler: TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.80-86
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
This study developed and evaluated a nanomaterial-modified ELISA system to analyze a peptide biomarker (Human VEGF). Multiwalled carbon nanotubes (MWCNTs) were used as nanomaterial-based modifiers for the capture/detection antibodies and HRP enzyme. CNTs modifications of the ELISA method at these three different binding steps were tested and compared. The most efficient modification step was concluded depending on these works’ sensitivities, linearities, and repeatabilities. We proved that CNTs could be used successfully to enhance the performances compared to the traditional ELISA method by achieving higher sensitivities and, therefore, more confident results, especially with a 0.5 pg/ml the limit of detection value. When comparing the three modification steps of CNTs binding, we observed the most sensitive results with the CNTs/detection antibody complex usage. Improved results (sensitivity is doubled compared to the test when no CNTs were used), improved reaction with serum samples, and good stability compared to the unmodified conventional ELISA system were obtained when CNTs were utilized with the detection antibodies as signal enhancers.