Research Information System
Microbes and Infectious Diseases, vol.6, no.3, pp.3065-3074, 2025 (Scopus)
| Abstract | |
| Background: Helicobacter pylori is a pathogen involved in the etiopathogenesis of gastroduodenal infections and some gastric malignancies. Accurate diagnosis and rapid detection of antibiotic resistance are crucial for effective treatment. Methods: We investigated the efficacy of a novel, multiplex, real-time PCR (qPCR) kit (DiaRD-HPykl, Diagen, Turkey) to detect H. pylori on 181 gastric biopsy specimens and simultaneously search clarithromycin resistance. qPCR results obtained from 49 fresh and 132 paraffinized gastric biopsy specimens were compared with histopathologic examination (HPE). Results: qPCR was positive in 89.2% and 74.2% of the fresh and paraffinized samples with HPE positive result, respectively. These values were 41.7% and 5.7% for HPE negative samples. The overall agreement between HPE and qPCR was 80.1% and the Kappa coefficient of agreement was 0.551. In fresh tissues, the actual agreement was 81.6% and the Kappa value was 0.489, while in paraffinized tissues, these values were 79.6% and 0.566, respectively. Clarithromycin resistance was detected in 12.5% of 112 samples, which were H. pylori positive by qPCR. Conclusion: The DiaRD-Hpykl qPCR kit, which is resulted in two hours, can be used in combination with HPE or alone as an alternative test for rapid diagnosis and accurate management of treatment. |