The current study aimed to detect of circulating tumor cells (CTCs) in 7.5 mL blood sample in bladder cancer patients treated with
transurethral resection (TUR) or radical cystectomy (RC) and compare the pre-operative and post-operative CTC counts.
Materials and Methods
CTCs were detected in peripheral blood of 10 bladder cancer patients with flow cytometry using biomarkers EpCAM, CK 14, 15, 16,
19 and CK 7, 8 for positive selection and CD45 for negative selection. The blood samples obtained before and after operation were
studied in 5 out of 10 patients.
The CTC counts decreased significantly in patients (Z=2.032; p=0.042) after the operation. The pre-operative CTC counts for the
patient group were found to be significantly higher than the control group. No CTCs were detected in the control group.
The results are promising in terms of CTC detection in bladder cancer (Bca) by flow cytometry. Pre- and post-operative CTC counts
as predictive and prognostic biomarker dramatically change in this study.
Bladder cancer (Bca); Circulating tumor cells (CTCs); Flow cytometry.