Electrochemical Enzymatic Biosensor Development by Using Different Electropolymerization Conditions of Polyaniline
5th International Marmara Sciences Congress (Imascon 2020), Kocaeli, Türkiye, 4 - 05 Aralık 2020, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Kocaeli
- Basıldığı Ülke: Türkiye
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
Polyaniline (PANI) is one of the most preferred and known conductive polymers for biosensor design
in view of its excellent electrochemical properties. PANI is an efficient conducting platform for
biosensor design because of its excessive redox behavior and its ability to mediate the electron transfer
between the reaction site and the electrode surface. In this research, an electrochemical enzymatic
biosensor system was developed with PANI film coated screen printed electrodes (SPE) using the
direct electropolymerization (one-step) process. PANI electropolymerization was performed in
different acidic solutions, and the effects on the electrodeposition of the potential range, potential scan
rate, and cycle number are discussed. The surface morphologies of films prepared with different
processes were characterized by the scanning electron microscopy (SEM) technique. A highly stable
and effective catechol biosensor was prepared by immobilizing tyrosinase (Tyr) enzyme into PANI
film in conjunction with cross-linking with glutaraldehyde. After optimizing the biosensor
performance conditions, the developed biosensor was used to quantify catechol in green tea samples.