ELECTROCHEMICAL ENZYMATIC BIOSENSOR DEVELOPMENT BY USING DIFFERENT ELECTROPOLYMERIZATION CONDITIONS OF POLYANILINE


Yıldırım Tirgil N.

International Marmara Sciences Congress (AUTUMN 2020), Kocaeli, Turkey, 4 - 05 December 2020, pp.155-163

  • Publication Type: Conference Paper / Full Text
  • City: Kocaeli
  • Country: Turkey
  • Page Numbers: pp.155-163

Abstract

Polyaniline (PANI) is one of the most preferred and known conductive polymers for biosensor design in view of its excellent electrochemical properties. PANI is an efficient conducting platform for biosensor design because of its excessive redox behavior and its ability to mediate the electron transfer between the reaction site and the electrode surface. In this research, an electrochemical enzymatic biosensor system was developed with PANI film coated screen printed electrodes (SPE) using the direct electropolymerization (one-step) process. PANI electropolymerization was performed in different acidic solutions, and the effects on the electrodeposition of the potential range, potential scan rate, and cycle number are discussed. The surface morphologies of films prepared with different processes were characterized by the scanning electron microscopy (SEM) technique. A highly stable and effective catechol biosensor was prepared by immobilizing tyrosinase (Tyr) enzyme into PANI film in conjunction with cross-linking with glutaraldehyde. After optimizing the biosensor performance conditions, the developed biosensor was used to quantify catechol in green tea samples.