© 2021 Wiley-VCH GmbHIn this research, an electrochemical enzymatic biosensor system for sensitive and selective catechol detection was developed with using boric acid substituted Polyaniline film coated two different electrodes; gold screen-printed electrode and glassy carbon electrode. Electropolymerization of 3-aminophenylboronic acid is carried out through various electropolymerization conditions, and the best conditions for biosensors systems are concluded. The developed tyrosinase enzyme-based biosensor system is used for the determination of catechol. The limit of detections(LOD) of the developed biosensor was 0.25 μM and 1.8 μM with Au SPE and GCE systems, respectively. Additionally, real sample analysis was also performed for controlled catechol added green tea samples.