A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin.


NEŞELİOĞLU S. , Ergin M. , EREL Ö.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, vol.33, no.12, pp.1339-1344, 2017 (Journal Indexed in SCI Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 12
  • Publication Date: 2017
  • Doi Number: 10.2116/analsci.33.1339
  • Title of Journal : Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
  • Page Numbers: pp.1339-1344

Abstract

A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001), norfloxacine oxidase (r = 0.989, p <0.001) and nephelometric methods (r = 0.861, p <0.001). This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.