A New Kinetic, Automated Assay to Determine the Ferroxidase Activity of Ceruloplasmin


NEŞELİOĞLU S. , Ergin M., EREL Ö.

ANALYTICAL SCIENCES, cilt.33, ss.1339-1344, 2017 (SCI İndekslerine Giren Dergi)

  • Cilt numarası: 33 Konu: 12
  • Basım Tarihi: 2017
  • Doi Numarası: 10.2116/analsci.33.1339
  • Dergi Adı: ANALYTICAL SCIENCES
  • Sayfa Sayısı: ss.1339-1344

Özet

A new kinetic and automated assay was developed to determine ceruloplasmin ferroxidase activity. Ferrous ions are turned into ferric ions via catalytic activity of the ferroxidase enzyme. Acetohydroxamic acid, a chromogen, forms a colored complex with ferric ions. This reaction was measured kinetically. Significant and strong correlations were obtained between the new acetohydroxamic method and the p-phenylenediamine oxidase (r = 0.988, p <0.001), o-dianisidine oxidase (r = 0.981, p <0.001), norfloxacine oxidase (r = 0.989, p <0.001) and nephelometric methods (r = 0.861, p <0.001). This reliable, applicable, user-friendly, and low-priced method can be performed fully automatically or with manual spectrophotometry, and can be used to measure the ferroxidase activity of ceruloplasmin.