The genetic polymorphisms of Eustigmaeus erciyesiensis Dogan, Ayyildiz & Fan (Acari: Stigmaeidae) populations collected from Ahmediye and Eksisu areas in Erzincan were determined by RAPD-PCR method (Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction). The genetic distances among the populations were shown with dendrogram and the dimensions of intra-specific variations were brought out. The random primers, OPAA08, OPAA19, OPAB01, OPAB05, OPAB18, OPAC09, OPAC17, OPAC19, OPAD10, OPAE09, OPAE12 and OPAE17 were used in the experiment. We obtained 134 bands in varying size from 100 to 4000 base pairs. Total polymorphism rate was 55.2 %. As a result, it was determined that intra-species genetic variations in the populations of E. erciyesiensis are high. With this study, E. erciyesiensis has been analysed at molecular level for the first time.