The aim of this study was to compare the cyclic fatigue resistance of rotary NiTi files, produced with four different manufacturing methods on specially designed dynamic models that simulated clinical conditions. In this study, 120 files, consisting of 30 files for Typhoon, ProTaper Next, RaCe, and Twisted Files nickel titanium rotary systems were used. The 30 files of each group were divided into three subgroups to be used in artificial canals with a 60 degrees angle of curvature and radii of curvature of 2, 5, and 8 mm (n = 10). All files were rotated in the artificial canals until fracture occurred and the number of cycles to fracture was calculated. The data were analyzed using one- and two-way analyses of variance and Tamhane multiple comparison tests. In all three groups, Typhoon instruments had the highest number of cycles to failure than the RaCe, ProTaper Next, and Twisted Files instruments, and the difference statistically significant (p < .05). There were no significant differences between the RaCe, ProTaper Next, and Twisted Files groups (p > .05). The CM wire Typhoon system was significantly more resistant to cyclic fatigue compared to the other file systems in all three artificial canals. When the fracture resistance of an instrument in three different artificial canals was compared, the mean NCFs decreased as the radius of the curvature of the canal decreased from 8 to 2 mm. Manufacturing method is one of the most important factors on cyclic fatigue resistance, also the radius of curvature effects the cyclic fatigue.