A colorimetric method to measure oxidized, reduced and total glutathione levels in erythrocytes

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Alisik M., Neselioglu S., Erel Ö.

JOURNAL OF LABORATORY MEDICINE, vol.43, no.5, pp.269-277, 2019 (SCI-Expanded, Scopus)

• Publication Type: Article / Article
• Volume: 43 Issue: 5
• Publication Date: 2019
• Doi Number: 10.1515/labmed-2019-0098
• Journal Name: JOURNAL OF LABORATORY MEDICINE
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Page Numbers: pp.269-277
• Open Archive Collection: AVESIS Open Access Collection
• Ankara Yıldırım Beyazıt University Affiliated: No

Abstract

Background: The most important antioxidant for erythrocytes is glutathione. In this study, a non-enzymatic recycling spectrophotometric method was developed to measure oxidized and reduced glutathione (GSH) levels in erythrocytes. The newly developed method consists of two steps. In the first step, the levels of GSH were determined in the sample. Subsequently, total glutathione levels were measured by reducing the oxidized glutathione (GSSG) with sodium borohydride (NaBH4). Half of the difference between total glutathione and GSH gives the GSSG.