The aim of this study was to investigate dynamic thiol-disulphide homeostasis as a novel oxidative stress parameter in migraine patients. A total of 115 participants were included in the study, and 63 of whom were diagnosed with migraine. The total thiol (-SH+-S-S-) and native thiol (-SH) levels in the serum were measured in all patients and healthy individuals. The dynamic disulphide bond (-S-S-) and (-S-S-) x 100/(-SH), (-S-S-) x 100/(-SH+-S-S-), and -SH x 100/(-SH+-S-S-) ratios were calculated from these values. The total and native thiol levels of migraine patients participating in the study were found to be significantly higher than the total and native thiol levels of healthy individuals. No statistically significant difference was determined in terms of the dynamic disulphide bond amounts or (-S-S-) x 100/(-SH), (-S-S-) x 100/(-SH+-S-S-), and -SH x 100/(-SH+-S-S-) ratios. The total thiol, native thiol, and dynamic disulphide bond levels, and (-S-S-) x 100/(-SH), (-S-S-) x 100/(-SH+-S-S-), and -SH x 100/(-SH+-S-S-) ratios were not correlated with attack frequency, pain intensity, or migraine type. Oxidative stress is considered to be one of the molecular changes underlying the pathogenesis of migraine.