The effect of simvastatin treatment on TNF-alpha–induced inflammation in human vascular endothelial cells (HUVECs)
Turkish Journal of Veterinary and Animal Sciences, cilt.47, sa.3, ss.236-247, 2023 (SCI-Expanded, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 47 Sayı: 3
- Basım Tarihi: 2023
- Doi Numarası: 10.55730/1300-0128.4291
- Dergi Adı: Turkish Journal of Veterinary and Animal Sciences
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, Veterinary Science Database, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.236-247
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Ankara Yıldırım Beyazıt Üniversitesi Adresli: Evet
Özet
This study investigates the effects of simvastatin (SMV) on human umbilical vein endothelial cells (HUVECs) inflamed by tumor necrosis factor-alpha (TNF-a). Cells were treated with simvastatin (5 μM, 10 μM, and 50 μM) with/without TNF (20 ng/mL). The effects of simvastatin on the proliferation of cells were examined, and its cytotoxic effect was determined by lactate dehydrogenase (LDH) assays. Metalloproteinase expression (MMP-2 and MMP-9) levels of SMV at different concentrations were determined by qPCR, and protein levels were determined by Western blot. The increasing concentrations of SMV down-regulated the expression levels of MMPs. After stimulation with TNF-a, the effect of SMV at different concentrations on wound healing time in case of wound formation was examined. The results interestingly indicated that SMV delayed the closure time 2.5-fold when it was administered alone to HUVECs but did not change the wound closure time in inflamed conditions. The study suggests that inflammation is a required condition in wound healing, and in the absence of inflammation, SMV treatment delays wound healing in vitro.