© 2020 SAABThe use of herbal medicines has become popular globally because of their efficacy and safety. Sternbergia species are known as imperative medicinal plants due to their alkaloid contents. In this study, we aimed to investigate the antioxidant, anti-inflammatory and antidiabetic activities of the ethanol and aqueous extracts from the bulbs of Sternbergia lutea ssp. lutea and Sternbergia lutea ssp. sicula. The antioxidant potentials of the extracts were evaluated by DPPH and ABTS radical scavenging activities. Measurement of anti-inflammatory activity was performed by heat induced hemolysis of human red blood cell membrane whereas antidiabetic activity by inhibition of in vitro α-glucosidase. It has been determined that the aqueous and ethanol extracts of Sternbergia lutea ssp.sicula and Sternbergia lutea ssp.lutea possessed low antioxidant and α-glucosidase inhibitory activity. This could be explained by low phenolic contents and different extraction procedures. In present study, we studied on maserates of two Sternbergia species similar forms used in traditional medicine, not a fraction of extracts rich in any chemical compounds. Ethanol extracts of Sternbergia lutea ssp. sicula possessed the strongest DPPH and ABTS scavenging activity with the IC50 value of 0.37 mg/ml and 0.10 mg/ml, respectively. Aqueous extracts of Sternbergia lutea ssp.sicula observed the highest effect on inhibiting α-glucosidase (IC50=0.01 mg/ml). Sternbergia lutea ssp. lutea aqueous extracts exhibited higher in vitro anti-inflammatory effect (IC50=8.02 mg/ml) than the other extracts. These results suggest that aqueous extracts were found to possess more potent anti-inflammatory and antidiabetic activity. However, further studies are needed for the isolation and identification of the effective compounds from the extracts of Sternbergia lutea ssp. lutea and Sternbergia lutea ssp. sicula.