Reusable Turn-on Fluorescent Biosensor for Cardiac Biomarker Troponin I Detection Using QDs-SPION-Aptamer

Ozdemir A., Algül Y., YILDIRIM TİRGİL N.

Journal of Fluorescence, 2025 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2025
  • Doi Number: 10.1007/s10895-025-04303-0
  • Journal Name: Journal of Fluorescence
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chimica, Compendex, MEDLINE
  • Keywords: Aptamer, Fluorescence, Quantum dots, Superparamagnetic iron oxide nanoparticles
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

Tro ponin is a biomolecule found in the blood of humans that can be used as an indicator of cardiac damage and cardiovascular risk. In this study, a turn-on fluorescence-based biosensor was developed using CdSe/ZnS quantum nanoparticles with high fluorescence efficiency decorated on superparamagnetic nanoparticles and aptamer as a probe for cardiac troponin I detection to improve fluorescence efficiency and create a reusable sensor through magnetic separation. Chemical modifications of biosensor components have been characterized by zeta sizer, zeta potential, UV–Vis spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). We reported that the developed sensor worked with a "Turn-on" mechanism. Fluorescence changes of the developed aptamer-based biosensor were evaluated using a fluorometer, and a low limit of detection (LOD) of 13.3 ng/ml with a linear detection range from 2 to 40 ng/ml was determined. Additionally, the selectivity and sensitivity of the biosensor have been tested with several biomarkers found in human blood.