Multiplexed cell-based diagnostic devices for detection of renal biomarkers


Köse S., Ahan R. E., Köksaldı İ. Ç., Olgaç A., KASAPKARA Ç. S., Şeker U. Ö. Ş.

Biosensors and Bioelectronics, vol.223, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 223
  • Publication Date: 2023
  • Doi Number: 10.1016/j.bios.2022.115035
  • Journal Name: Biosensors and Bioelectronics
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Synthetic biology, Whole cell sensors, Urea, Uric acid
  • Ankara Yıldırım Beyazıt University Affiliated: No

Abstract

© 2022 Elsevier B.V.The number of synthetic biology-based solutions employed in the medical industry is growing every year. The whole cell biosensors being one of them, have been proven valuable tools for developing low-cost, portable, personalized medicine alternatives to conventional techniques. Based on this concept, we targeted one of the major health problems in the world, Chronic Kidney Disease (CKD). To do so, we developed two novel biosensors for the detection of two important renal biomarkers: urea and uric acid. Using advanced gene expression control strategies, we improved the operational range and the response profiles of each biosensor to meet clinical specifications. We further engineered these systems to enable multiplexed detection as well as an AND-logic gate operating system. Finally, we tested the applicability of these systems and optimized their working dynamics inside complex medium human blood serum. This study could help the efforts to transition from labor-intensive and expensive laboratory techniques to widely available, portable, low-cost diagnostic options.