ASSOCIATION BETWEEN HER2/NEU STATUS IN COLORECTAL CARCINOMA AND CLINICOPATHOLOGICAL FEATURES: A RETROSPECTIVE STUDY USING WHOLE - TISSUE SECTIONS


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Kilicarslan A., Dogan H. T. , Sungu N., Dogan M., Yalcin A., Dede D. S.

POLISH JOURNAL OF PATHOLOGY, cilt.69, ss.143-149, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 69 Konu: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.5114/pjp.2018.75671
  • Dergi Adı: POLISH JOURNAL OF PATHOLOGY
  • Sayfa Sayıları: ss.143-149

Özet

The human epidermal growth factor receptor 2 (Her2/neu) signal pathway plays a significant role in the occurance of various solid tumor types. The rate of Her2/neu in colorectal carcinoma (CRC) is not clearly elucidated. In this study, we discuss the association between Her2/neu overexpression and clinicopathological parameters in CRC. Her2/neu immunohistochemical (IHC) staining was performed on whole sections of formalin fixed paraffin embedded tumor tissues of 100 CRC resections. Cases with score 3+ and score 2+ expressions were further evaluated by silver in-situ hybridization (ISH) for the existence of Her2/neu gene amplification. Her2/neu membranous overexpression was observed in 12 of the 100 cases (6 cases with a score 3+ and 6 cases with a score 2+) and 6 of these were heterogenous. There were 10 cases with Her2/neu amplification (6/6 score 3+, 4/6 score 2 +). Strong cytoplasmic staining was observed in 5 cases. Membranous scores were either 3+ or 2+ in 3 of these 5 cases. Moreover, there was Her2/neu amplification in 2 of these 3 cases. Her2/neu amplification status and overexpression was not related to clinicopathological parameters and overall survival. More clear results can be obtained from studies with Her2/neu IHC and ISH test on whole sections.