Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis


Dal T., Kara S. S. , Cikman A., Balkan C. E. , Acikgoz Z. , Zeybek H., ...More

Journal of Infection and Public Health, vol.12, no.3, pp.337-342, 2019 (Journal Indexed in SCI Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 12 Issue: 3
  • Publication Date: 2019
  • Doi Number: 10.1016/j.jiph.2018.11.008
  • Title of Journal : Journal of Infection and Public Health
  • Page Numbers: pp.337-342

Abstract

Objective: Brucellosis is a zoonotic disease with various clinical presentations and early diagnosis is crucial to avoid severe complications. Due to limitations of conventional diagnostic methods, polymerase chain reaction (PCR) based approaches have gained importance in diagnosis. We aimed to evaluate diagnostic value of multiplex real time-PCR (mRT-PCR) in serum samples collected from brucellosis suspected patients by comparision sensitivity of mRT-PCR with those of conventional diagnostic methods.