Comparison of multiplex real-time polymerase chain reaction with serological tests and culture for diagnosing human brucellosis

Dal T., Kara S. S. , Cikman A., Balkan C. E. , Acikgoz Z. , Zeybek H., ...Daha Fazla

Journal of Infection and Public Health, cilt.12, ss.337-342, 2019 (SCI Expanded İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 12 Konu: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1016/j.jiph.2018.11.008
  • Dergi Adı: Journal of Infection and Public Health
  • Sayfa Sayıları: ss.337-342


Objective: Brucellosis is a zoonotic disease with various clinical presentations and early diagnosis is crucial to avoid severe complications. Due to limitations of conventional diagnostic methods, polymerase chain reaction (PCR) based approaches have gained importance in diagnosis. We aimed to evaluate diagnostic value of multiplex real time-PCR (mRT-PCR) in serum samples collected from brucellosis suspected patients by comparision sensitivity of mRT-PCR with those of conventional diagnostic methods.