Objective: There are not enough studies on the factors affecting chronic constipation, which is common worldwide. There- fore, the aim of this study was to compare physical activity level, sit- ting time and perceived stress level in women with and without chronic constipation. Material and Methods: Patients with (constipation group, n=29, age=30.72±5.98 years) and without (control group, n=31, age=31.13±6.75 years) chronic constipation according to the Rome IV diagnostic criteria were included in the study. Bowel functions were evaluated with the bowel diary (frequency of defecation, defecation du- ration, feeling of incomplete defecation, pain) and Bristol Stool Scale (stool type), physical activity level and sitting time were evaluated with the International Physical Activity Questionnaire-Short Form (IPAQ- SF), and perceived stress level was evaluated with the Perceived Stress Scale. Evaluations were carried out once. Results: The frequency of defecation was found to be lower in the constipation group compared to the control group, and the defecation duartion and the feeling of incom- plete defecation were higher than the control group (p<0.05). Stool type was found to be hard in the constipation group and normal in the con- trol group. It was observed that IPAQ-SF scores were lower (p=0.003) and sitting time was longer (p<0.001) in the constipation group com- pared to the control group. Perceived stress level was found to be higher in the constipation group than in the control group (p=0.023). Conclu- sion: It was found that women with chronic constipation had less phys- ical activity level, sitting time and perceived stress level more than women without constipation. According to these results, physical activ- ity, sitting time and perceived stress level should be considered in both assessment and treatment programs in constipation management.