Role Of Adamts-1 In Pleomorphic Xanthoastrocytoma Tumor Cells Progression


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Gokce A., Gokce E. C. , SUNGUROĞLU A.

Turkish Neurosurgery, vol.31, no.5, pp.731-739, 2021 (Journal Indexed in SCI Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.5137/1019-5149.jtn.31011-20.3
  • Title of Journal : Turkish Neurosurgery
  • Page Numbers: pp.731-739
  • Keywords: Glioma, Matrix Metalloproteases, NF-kappa B, Pleomorphic Xanthoastrocytoma, STAT3 transcription factor

Abstract

© 2021,Turkish Neurosurgery.All Rights Reserved.AIM: To analyze the expression of ADAMTS-1, NF-κB, and STAT3 in human pleomorphic xanthoastrocytoma specimens, and their correlation with glioma advancement. MATERIAL and METHODS: Pleomorphic xanthoastrocytoma tumor cell lines were treated with low and high doses of cytokines at 24 and 48 hours (h) to replicate the inflammatory environment. The effects of IL-1 were assessed with the scratch wound-healing assay, and the expression levels of ADAMTS-1, NF-κB, and STAT3 of the groups were determined by western blot analysis. RESULTS: Cytokine treatment significantly increased the migration of PXA glioma cells after scratching at 24h and 48h time points. Similarly, 10 and 30 ng/mL IL-1 induced 1.86 and 1.94 fold increases, respectively, in ADAMTS-1 expression after 24h, and 3 and 3.27 fold increases, respectively, after 48h, compared with the non-treatment control group.10 and 30 ng/mL IL-1 doses caused 2.5 and 2.6 fold increase, in NF-κB protein levels after 24h, and 3.16 and 3.41 fold increases after 48h, compared with the non-treatment group. The protein levels of STAT3 after 24h were 2.62 and 2.43 fold higher, and 3.78 and 3.84 fold higher after 48 hours, with 10 and 30 ng/mL IL-1, compared with the non-treatment group. CONCLUSION: The proliferation and progression of glioma cells were proportional to the increased expression levels of ADAMTS-1, NF-κB, and STAT3. Our findings indicate that the proteolytic function of ADAMTS-1 may be associated with the malignant transformation of low-grade gliomas.