Determination of homeodomain interacting protein kinase 2 polymorphisms rs2058265, rs6464214, and rs7456421 in patients with kidney stone Determinación de los polimorfismos rs2058265, rs6464214 y rs7456421 de la proteína cinasa 2 que interactúan con el homeodominio en pacientes con cálculos renales

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Ceylan C., Gokhan-Doluoglu O., Taştemur S., Keleş I., Ceylan G., Cevdet-Ceylan A. C., ...More

Cirugia y Cirujanos (English Edition), vol.91, no.4, pp.474-478, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 91 Issue: 4
  • Publication Date: 2023
  • Doi Number: 10.24875/ciru.22000472
  • Journal Name: Cirugia y Cirujanos (English Edition)
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, Directory of Open Access Journals
  • Page Numbers: pp.474-478
  • Keywords: Allele, HIPK2 gene, Kidney, Nephrolithiasis, Polymorphism, Stone
  • Ankara Yıldırım Beyazıt University Affiliated: Yes

Abstract

Objective: This study aimed to investigate whether homeodomain interacting protein kinase 2 (HIPK2) polymorphism is associated with renal stone formation in a Turkish population. Materials and methods: A total of 129 patients with calcium nephrolithiasis and 67 sex- and age-matched healthy controls were enrolled in the study. Blood samples were collected into EDTA tubes. The DNA of patients was extracted using a QIAsymphony® automated DNA isolation system. The Chi-square test was applied in the comparisons between the patient and control groups in respect of the differences in the genotype and allele frequencies. Results: No statistically significant difference was found between the groups in terms of single nucleotide polymorphism (SNP) incidence in single allele and double alleles in the rs2058265 and rs6464214 regions (p = 0.13 and 0.37, respectively). The SNP incidence in double alleles in nephrolithiasis patients at rs7456421 was statistically significantly lower than in the control group (p = 0.001). Conclusion: Distributions of the genotype and allele of the three polymorphisms (rs2058265, rs6464214, and rs745642 in HIPK2) were not associated with an increased risk of kidney stone in this Turkish population.